Soft Natto Manufactured with Starter of Bacillus subtilis KFP 843 Isolated from Douchi

In silico analysis of Nattokinase from Bacillus subtilis sp natto

Nattokinase or subtilisin NAT (EC 3.4.21.62) is one of the most remarkable enzymes produced by Bacillus subtilis sp. Natto, which posses direct fibrinolytic activity. The aim of this study is in silico analysis of Nattokinase structure and function. The three-dimensional structure of serine protease Nattokinase from Bacillus subtilis sp. natto was determined using homology modeling performed by...

Amyloid-degrading ability of nattokinase from Bacillus subtilis natto.

More than 20 unrelated proteins can form amyloid fibrils in vivo which are related to various diseases, such as Alzheimer's disease, prion disease, and systematic amyloidosis. Amyloid fibrils are an ordered protein aggregate with a lamellar cross-beta structure. Enhancing amyloid clearance is one of the targets of the therapy of these amyloid-related diseases. Although there is debate on whethe...

Comparative Fibrinolytic Activities of Nattokinases from Bacillus subtilis var. natto

Nattokinase is a potent fibrinolytic enzyme which was first found in a traditional Japanese soybean food. Two Nattokinase genes were cloned using chromosomal DNA from Bacillus subtilis (Ehrenberg 1835) Cohn 1872. Recombinant Natto-1.3 kb (GenBank accession number KF734090) and Natto-1.1 kb were expressed using a pQE-30 expression vector, and their fibrinolytic activities were studied. Natto-1.3...

Construction of physical maps of Bacillus subtilis (natto) strains.

The putative physical map of a Bacillus subtilis (natto) strain has been constructed to demonstrate the difference from the strain B. subtilis Marburg. B. subtilis (natto) strains are closely related to the Marburg 168trpC2 strain whose genome was sequenced. Their genome size and organization are also assumed to be similar. The putative SfiI and I-CeuI physical map of a natto strain BEST195 rev...

Examining Some Probiotics Activities of Bacillus Subtilis Natto